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Appl Environ Microbiol. 2011 Apr;77(8):2727-33. doi: 10.1128/AEM.02454-10. Epub 2011 Mar 4.

Metabolic engineering of Clostridium cellulolyticum for production of isobutanol from cellulose.

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  • 1Department of Chemical and Biomolecular Engineering, University of California, Los Angeles, 5531 Boelter Hall, 420 Westwood Plaza, Los Angeles, CA 90095, USA.

Erratum in

  • Appl Environ Microbiol. 2012 Oct;78(19):7171.


Producing biofuels directly from cellulose, known as consolidated bioprocessing, is believed to reduce costs substantially compared to a process in which cellulose degradation and fermentation to fuel are accomplished in separate steps. Here we present a metabolic engineering example for the development of a Clostridium cellulolyticum strain for isobutanol synthesis directly from cellulose. This strategy exploits the host's natural cellulolytic activity and the amino acid biosynthesis pathway and diverts its 2-keto acid intermediates toward alcohol synthesis. Specifically, we have demonstrated the first production of isobutanol to approximately 660 mg/liter from crystalline cellulose by using this microorganism.

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