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Trends Biotechnol. 2011 May;29(5):240-50. doi: 10.1016/j.tibtech.2011.01.007. Epub 2011 Mar 4.

Point-of-care nucleic acid testing for infectious diseases.

Author information

1
Keck Graduate Institute of Applied Life Sciences, 535 Watson Drive, Claremont, CA 91711, USA. aniemz@kgi.edu

Abstract

Nucleic acid testing for infectious diseases at the point of care is beginning to enter clinical practice in developed and developing countries; especially for applications requiring fast turnaround times, and in settings where a centralized laboratory approach faces limitations. Current systems for clinical diagnostic applications are mainly PCR-based, can only be used in hospitals, and are still relatively complex and expensive. Integrating sample preparation with nucleic acid amplification and detection in a cost-effective, robust, and user-friendly format remains challenging. This review describes recent technical advances that might be able to address these limitations, with a focus on isothermal nucleic acid amplification methods. It briefly discusses selected applications related to the diagnosis and management of tuberculosis, HIV, and perinatal and nosocomial infections.

PMID:
21377748
PMCID:
PMC3746968
DOI:
10.1016/j.tibtech.2011.01.007
[Indexed for MEDLINE]
Free PMC Article
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