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Microbes Infect. 2011 Jul;13(7):684-90. doi: 10.1016/j.micinf.2011.02.006. Epub 2011 Mar 2.

Detection of a geographical and endemic cluster of hyper-invasive meningococcal strains.

Author information

1
National Reference Centre for Neisseria meningitidis, Bacterial Diseases Division, Communicable and Infectious Diseases, Scientific Institute of Public Health, J. Wytsman Street, 14-16, B-1050 Brussels, Belgium. sophie.bertrand@wiv-isp.be

Abstract

From 2006 to December 2009, 45 out of the 513 strains isolated from patients with invasive meningococcal disease in Belgium, were identified as Neisseria meningitidis serogroup B, non-serotypeable, subtype P1.14 (B:NT:P1.14). Most cases were geographically clustered in the northern part of the country. Multilocus Sequence Typing and antigen gene sequencing combined with Pulsed-Field Gel electrophoresis were used to investigate this cluster. Molecular typing showed that 39 out of these 45 N. meningitidis strains belonged to the clonal complex cc-269. The presence of the same PorA Variable Regions (VR1-VR2: 22, 14), the FetA allele (F5-1) and the highly similar Pulsed-Field Gel Electrophoresis profiles, supported genetic relatedness for 38 out of these 39 isolates. Retrospective analysis of B:NT:P1.22,14 isolates from 1999 onwards suggested that these strains belonging to the cc-269 complex, first emerged in the Belgian province of West-Flanders in 2004. This study showed that the combination of molecular tools with classical methods enabled reliable outbreak detection as well as a cluster identification.

PMID:
21376133
DOI:
10.1016/j.micinf.2011.02.006
[Indexed for MEDLINE]

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