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Microb Biotechnol. 2011 Jul;4(4):503-12. doi: 10.1111/j.1751-7915.2010.00244.x. Epub 2011 Jan 17.

Butyrate production in phylogenetically diverse Firmicutes isolated from the chicken caecum.

Author information

1
Department of Pathology, Bacteriology and Avian Diseases, Research Group Veterinary Public Health and Zoonoses, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, Merelbeke, Belgium. venessa.eeckhaut@UGent.be

Abstract

Sixteen butyrate-producing bacteria were isolated from the caecal content of chickens and analysed phylogenetically. They did not represent a coherent phylogenetic group, but were allied to four different lineages in the Firmicutes phylum. Fourteen strains appeared to represent novel species, based on a level of ≤ 98.5% 16S rRNA gene sequence similarity towards their nearest validly named neighbours. The highest butyrate concentrations were produced by the strains belonging to clostridial clusters IV and XIVa, clusters which are predominant in the chicken caecal microbiota. In only one of the 16 strains tested, the butyrate kinase operon could be amplified, while the butyryl-CoA:acetate CoA-transferase gene was detected in eight strains belonging to clostridial clusters IV, XIVa and XIVb. None of the clostridial cluster XVI isolates carried this gene based on degenerate PCR analyses. However, another CoA-transferase gene more similar to propionate CoA-transferase was detected in the majority of the clostridial cluster XVI isolates. Since this gene is located directly downstream of the remaining butyrate pathway genes in several human cluster XVI bacteria, it may be involved in butyrate formation in these bacteria. The present study indicates that butyrate producers related to cluster XVI may play a more important role in the chicken gut than in the human gut.

PMID:
21375722
PMCID:
PMC3815262
DOI:
10.1111/j.1751-7915.2010.00244.x
[Indexed for MEDLINE]
Free PMC Article

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