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Mol Cell Biol. 1990 Mar;10(3):1249-53.

Phosphorylation of the ETS-2 protein: regulation by the T-cell antigen receptor-CD3 complex.

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Laboratory of Molecular Oncology, National Cancer Institute, Frederick, Maryland 21701-1013.


Phosphorylation of the human ets-2 protein in response to mitogenic signals to T lymphocytes was investigated in Jurkat cells. Activation of the cells by antibodies against the T-cell antigen receptor-CD3 complex or by concanavalin A was followed within 5 min by increased phosphorylation of the protein, as shown by a mobility shift of the protein from 54 to 56 kilodaltons in sodium dodecyl sulfate-polyacrylamide gel electrophoresis and increased incorporation of 32P. The Ca2+ ionophores A23187 and ionomycin were able to mimic this effect, suggesting that this phosphorylation is mediated by Ca2+.

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