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EMBO Mol Med. 2011 Apr;3(4):201-7. doi: 10.1002/emmm.201100129. Epub 2011 Mar 4.

Quantitative tracking of T cell clones after haematopoietic stem cell transplantation.

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1
Shemiakin-Ovchinnikov Institute of Bioorganic Chemistry, RAS, Moscow, Russia.

Abstract

Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA-based technology for unbiased amplification of T cell receptor beta-chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.

PMID:
21374820
PMCID:
PMC3377069
DOI:
10.1002/emmm.201100129
[Indexed for MEDLINE]
Free PMC Article
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