Format

Send to

Choose Destination
Nat Protoc. 2011 Mar;6(3):405-17. doi: 10.1038/nprot.2010.200. Epub 2011 Mar 3.

Dynamic clamp with StdpC software.

Author information

1
School of Life Sciences, University of Sussex, Brighton, UK.

Abstract

Dynamic clamp is a powerful method that allows the introduction of artificial electrical components into target cells to simulate ionic conductances and synaptic inputs. This method is based on a fast cycle of measuring the membrane potential of a cell, calculating the current of a desired simulated component using an appropriate model and injecting this current into the cell. Here we present a dynamic clamp protocol using free, fully integrated, open-source software (StdpC, for spike timing-dependent plasticity clamp). Use of this protocol does not require specialist hardware, costly commercial software, experience in real-time operating systems or a strong programming background. The software enables the configuration and operation of a wide range of complex and fully automated dynamic clamp experiments through an intuitive and powerful interface with a minimal initial lead time of a few hours. After initial configuration, experimental results can be generated within minutes of establishing cell recording.

PMID:
21372819
PMCID:
PMC3188375
DOI:
10.1038/nprot.2010.200
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center