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Opt Express. 2011 Feb 14;19(4):3130-43. doi: 10.1364/OE.19.003130.

Simultaneous multi-lifetime multi-color STED imaging for colocalization analyses.

Author information

1
Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Göttingen, Germany.

Abstract

We describe a STED microscope optimized for colocalization experiments with up to three colors. Two fluorescence labels are separated by their fluorescence lifetime whereas a third channel is discriminated by the wavelength of fluorescence emission. Since it does not require a second STED beam, separating by lifetime is insensitive to drift and thus optimally suited for colocalization analyses. Furthermore, we propose a setup having a second STED beam for long duration multicolor recording.

PMID:
21369135
DOI:
10.1364/OE.19.003130
[Indexed for MEDLINE]

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