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Genetics. 2011 May;188(1):229-33. doi: 10.1534/genetics.110.126193. Epub 2011 Mar 2.

Focusing transgene expression in Drosophila by coupling Gal4 with a novel split-LexA expression system.

Author information

1
Eunice Kennedy Shriver National Institute of Child Healthand Human Development, National Institutes of Health, Bethesda, MD 20892, USA.

Abstract

Here we report the development of a ternary version of the LexA::VP16/LexAop system in which the DNA-binding and trans-activating moieties are independently targeted using distinct promoters to achieve highly restricted, intersectional expression patterns. This Split LexA system can be concatenated with the Gal4/upstream activating sequence system to refine the expression patterns of existing Gal4 lines with minimal genetic manipulations.

PMID:
21368278
PMCID:
PMC3120155
DOI:
10.1534/genetics.110.126193
[Indexed for MEDLINE]
Free PMC Article

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