Format

Send to

Choose Destination
Cell Motil Cytoskeleton. 1990;15(1):34-40.

Epidermal growth factor induces the accumulation of calpactin II on the cell surface during membrane ruffling.

Author information

1
Cancer Research Center of Hawaii, University of Hawaii, Honolulu.

Abstract

Confluent and proliferatively quiescent T51B rat liver epithelial cells provide a cellular model for the study of epidermal growth factor (EGF) effects in non-neoplastic cells. Immunoreactive calpactin II, a well-known substrate for EGF-receptor kinase, was found predominantly in the cytosol, although a second immunoreactive pool was found in a Triton X-100-extractable membrane fraction. Stimulation with EGF resulted in a rapid and transient (2-5 min) formation of ruffles at the cell surface and at the cell-cell contacts. Both calpactin II and filamentous actin were found co-localized at the membrane ruffles. Immunoprecipitations of membrane-bound calpactin II from 32P-labeled cells indicate a transient EGF-dependent phosphorylation of calpactin II correlating with membrane ruffling. These results suggest a temporal (2-5 min) function for calpactin II at the plasma membrane during the EGF-induced mitogenesis of T51B cells.

PMID:
2136806
DOI:
10.1002/cm.970150106
[Indexed for MEDLINE]

Supplemental Content

Loading ...
Support Center