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Cell Death Dis. 2010 Apr 22;1:e36. doi: 10.1038/cddis.2010.14.

Protection of rat pancreatic islet function and viability by coculture with rat bone marrow-derived mesenchymal stem cells.

Author information

1
Stem Cell and Gene Therapy Research and Application Center, Kocaeli University, Kocaeli, Turkey. ekaraoz@hotmail.com

Abstract

The maintenance of viable and functional islets is critical in successful pancreatic islet transplantation from cadaveric sources. During the isolation procedure, islets are exposed to a number of insults including ischemia, oxidative stress and cytokine injury that cause a reduction in the recovered viable islet mass. A novel approach was designed in which streptozotocin (STZ)-damaged rat pancreatic islets (rPIs) were indirectly cocultured with rat bone marrow-derived mesenchymal stem cells (rBM-MSCs) to maintain survival of the cultured rPIs. The results indicated that islets cocultured with rBM-MSCs secreted an increased level of insulin after 14 days, whereas non-cocultured islets gradually deteriorated and cell death occurred. The cocultivation of rBM-MSCs with islets and STZ-damaged islets showed the expression of IL6 and transforming growth factor-β1 in the culture medium, besides the expression of the antiapoptotic genes (Mapkapk2, Tnip1 and Bcl3), implying the cytoprotective, anti-inflammatory and antiapoptotic effects of rBM-SCs through paracrine actions.

PMID:
21364643
PMCID:
PMC3032304
DOI:
10.1038/cddis.2010.14
[Indexed for MEDLINE]
Free PMC Article

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