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J Biomed Opt. 2011 Feb;16(2):026014. doi: 10.1117/1.3540674.

Simplified approach for quantitative digital holographic phase contrast imaging of living cells.

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1
University of Muenster, Center for Biomedical Optics and Photonics, Robert-Koch-Str. 45, D-48149 Muenster, GermanyUniversity of Muenster, Gastroenterological Molecular Cell Biology, Department of Medicine B, D-48149 Muenster, Germany.

Abstract

Many interferometry-based quantitative phase contrast imaging techniques require a separately generated coherent reference wave. This results in a low phase stability and the demand for a precise adjustment of the intensity ratio between object and reference wave. To overcome these problems, the performance of a Michelson interferometer approach for digital holographic microscopy was analyzed that avoids a separately generated reference wave by superposition of different image areas. It is shown that this simplified arrangement yields improved phase stability. Furthermore, results from time-lapse investigations on living pancreas tumor cells demonstrate the capability of the method for reliable quantitative phase contrast imaging.

PMID:
21361698
DOI:
10.1117/1.3540674
[Indexed for MEDLINE]
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