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J Biomed Opt. 2011 Feb;16(2):021103. doi: 10.1117/1.3490641.

Forward-collected simultaneous fluorescence lifetime imaging and coherent anti-Stokes Raman scattering microscopy.

Author information

1
National Research Council of Canada, Steacie Institute for Molecular Sciences, Ottawa, Ontario, K1A 0R6 Canada. slepkov@gmail.com

Abstract

We demonstrate the simultaneous collection and separation of femtosecond-laser-based forward-collected coherent anti-Stokes Raman scattering (F-CARS) and two-photon-excitation-induced fluorescence lifetime images (FLIM) using time-correlated single photon counting (TCSPC). We achieve this in a nondescanned geometry using a single multimode fiber without significant loss of light, field of view, and most importantly, TCSPC timing fidelity. In addition to showing the ability to separate CARS images from FLIM images using time gating, we also demonstrate composite multimodal epicollected FLIM imaging with fiber-collected F-CARS imaging in live cells.

PMID:
21361666
DOI:
10.1117/1.3490641
[Indexed for MEDLINE]
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