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CSH Protoc. 2007 May 1;2007:pdb.prot4723. doi: 10.1101/pdb.prot4723.

Cell Preparation and Multicolor FISH in 3D Preserved Cultured Mammalian Cells.

Author information

1
Ludwig-Maximilians University Munich, Department of Biology II, AG Thomas Cremer (Chair of Anthropology and Human Genetics), 82152 Martinsried-Planegg, Germany.

Abstract

INTRODUCTIONFluorescence in situ hybridization (FISH) on three-dimensional preserved nuclei (3D-FISH) in combination with three-dimensional-microscopy and image reconstruction is an efficient tool to analyze the arrangement of distinct nuclear targets such as entire chromosome territories, chromosomal subregions, or single gene loci on a single-cell level. This protocol focuses on fixation, pretreatments, and 3D-FISH on cultured mammalian cells. It can be applied to a variety of cell types growing adherently or in suspension.

PMID:
21357073
DOI:
10.1101/pdb.prot4723

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