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CSH Protoc. 2007 Jan 1;2007:pdb.prot4643. doi: 10.1101/pdb.prot4643.

Denaturing protein immunoprecipitation from yeast.

Author information

1
Cold Spring Harbor Laboratory, Cold Spring Harbor, NY 11724, USA.

Abstract

INTRODUCTIONWhere possible, it is preferable to use denaturing IPs to recover labeled proteins from pulse-chase experiments. The use of denaturing IPs reduces background and simplifies protein harvest. Yeast are boiled in an SDS-containing solution to liberate total proteins. This is also much safer than bead-beating radioactive yeast samples.

PMID:
21356925
DOI:
10.1101/pdb.prot4643

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