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CSH Protoc. 2008 Oct 1;2008:pdb.prot5054. doi: 10.1101/pdb.prot5054.

Gene knockdown in planarians using RNA interference.

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Center for Regenerative and Developmental Biology, Forsyth Institute and Developmental Biology Department, Harvard School of Dental Medicine, Boston, MA 02115, USA.


INTRODUCTIONThis protocol describes how to produce gene knockdown in planarians using RNA interference (RNAi). It is a standard technique to evaluate gene function during regeneration and tissue maintenance in planarians. The procedure involves microinjecting double-stranded RNA (dsRNA) synthesized in vitro. Depending on the gene target, this technique can produce robust phenotypes that can be further evaluated by diverse macroscopic or microscopic procedures.


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