Format

Send to

Choose Destination
See comment in PubMed Commons below
Anal Chem. 2011 Mar 15;83(6):2145-51. doi: 10.1021/ac102978f. Epub 2011 Feb 25.

Nonresonant femtosecond laser vaporization with electrospray postionization for ex vivo plant tissue typing using compressive linear classification.

Author information

1
Temple University, Department of Chemistry, 1901 North 13th Street, Philadelphia, Pennsylvania 19122, United States.

Abstract

Laser electrospray mass spectrometry (LEMS) with offline classification is used to discriminate plant tissues at atmospheric pressure using an intense (10(13) W cm(-2)), nonresonant (800 nm) femtosecond laser pulse to vaporize cellular content for subsequent mass analysis. The tissue content of the plant within the 0.05 mm(2) laser interaction region is vaporized into the electrospray plume where the molecules are ionized prior to transfer into the mass spectrometer. The measurements for a flower petal, leaf, and stem of an impatiens plant reveal mass spectral signatures that enable discrimination as performed using a compressive linear classifier. The statistical analysis of the plant tissue samples reveals reproducibility of the data for replicate tissue samples and within a single tissue sample. A similar degree of discrimination was achieved for the green and white regions of aphelandra squarrosa (zebra plant) leaves.

PMID:
21351794
DOI:
10.1021/ac102978f
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for American Chemical Society
    Loading ...
    Support Center