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Bioinformatics. 2011 Apr 15;27(8):1159-61. doi: 10.1093/bioinformatics/btr087. Epub 2011 Feb 23.

PrimerProspector: de novo design and taxonomic analysis of barcoded polymerase chain reaction primers.

Author information

1
Department of Molecular, Cellular, and Developmental Biology, Cooperative Institute for Research in Environmental Sciences, University of Colorado at Boulder, Boulder, CO 80309, USA.

Abstract

MOTIVATION:

PCR amplification of DNA is a key preliminary step in many applications of high-throughput sequencing technologies, yet design of novel barcoded primers and taxonomic analysis of novel or existing primers remains a challenging task.

RESULTS:

PrimerProspector is an open-source software package that allows researchers to develop new primers from collections of sequences and to evaluate existing primers in the context of taxonomic data.

AVAILABILITY:

PrimerProspector is open-source software available at http://pprospector.sourceforge.net

CONTACT:

rob.knight@colorado.edu

SUPPLEMENTARY INFORMATION:

Supplementary data are available at Bioinformatics online.

PMID:
21349862
PMCID:
PMC3072552
DOI:
10.1093/bioinformatics/btr087
[Indexed for MEDLINE]
Free PMC Article
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