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J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Mar 15;879(9-10):599-604. doi: 10.1016/j.jchromb.2011.01.022. Epub 2011 Feb 2.

Analysis of the interactions of multicomponents in Cornus officinalis Sieb. et Zucc. with human serum albumin using on-line dialysis coupled with HPLC.

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  • 1Key Laboratory of the Ministry of Education for Medicinal Plant Resources and Natural Pharmaceutical Chemistry, Shaanxi Normal University, Xi'an 710062, China.

Abstract

Interactions of three iridoid glycosides extracted from Cornus officinalis Sieb. et Zucc. (CIG) with protein were simultaneously explored by on-line dialysis sampling coupled with high-performance liquid chromatography (DS-HPLC). Three main compounds in CIG were unequivocally identified as loganin, sweroside and cornuside by comparing their t(R), MS data and UV spectra with those of reference compounds. Dialysis recoveries and quantitative characteristics of DS-HPLC for three iridoid glycosides were determined. Recoveries of dialysis sampling ranged from 73.9 to 91.7% with the RSD below 3.0%. Based on the determination of concentrations before and after interaction with human serum albumin (HSA), the binding parameters of loganin, sweroside and cornuside with HSA were obtained and the binding mechanisms were investigated.

PMID:
21345748
DOI:
10.1016/j.jchromb.2011.01.022
[PubMed - indexed for MEDLINE]
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