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Chromosome Res. 2011 Feb;19(2):235-49. doi: 10.1007/s10577-011-9193-8. Epub 2011 Feb 22.

The SUUR protein is involved in binding of SU(VAR)3-9 and methylation of H3K9 and H3K27 in chromosomes of Drosophila melanogaster.

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1
Department of Molecular and Cellular Biology, Institute of Chemical Biology and Fundamental Medicine, Lavrentiev Ave., 8, Novosibirsk, 630090, Russia. koryakov@mcb.nsc.ru

Abstract

In the present work, we found that the SUUR protein is required for the SU(VAR)3-9 enzyme to bind to the salivary gland polytene chromosomes. The SuUR mutation results in loss of SU(VAR)3-9 on the chromosomes, whereas artificial expression of the SuUR gene restores its binding. The SUUR protein is also involved in methylation of the residues H3K9 and H3K27. However, mono-, di-, and tri-methylated forms of H3K9 and H3K27 behave differently in various chromosomal domains in response to the SuUR mutation. Euchromatin and chromosome 4 are almost completely deprived of mono-, di-, and tri-methylation of H3K9. In the chromocenter, mono-methylation is reduced, di-methylation shows no noticeable changes, and tri-methylation is lost. Furthermore, mono- and di-methylation of H3K27 are not influenced by the SuUR mutation, whereas tri-methylation is lost in the chromocenter. Artificial expression of the SuUR gene on the SuUR (-) background restores the pattern of methylated residues characteristic for the wild type.

PMID:
21340745
DOI:
10.1007/s10577-011-9193-8
[Indexed for MEDLINE]
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