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Pept Res. 1990 Nov-Dec;3(6):274-6.

Colorimetric assay of HIV-1 proteinase suitable for high-capacity screening.

Author information

1
Sandoz Forschungsinstitut, Vienna, Austria.

Abstract

The cleavage of substrate peptides by HIV-1 proteinase can be monitored by reaction of exposed amino groups with 2,4,6-trinitrobenzenesulfonic acid to yield yellow-colored products. This method provides a high-capacity assay of the enzyme which can be performed on microtiter plates.

PMID:
2134071
[Indexed for MEDLINE]

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