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Int J Mol Sci. 2011 Jan 21;12(1):773-94. doi: 10.3390/ijms12010773.

MALDI Imaging Mass Spectrometry (MALDI-IMS)-application of spatial proteomics for ovarian cancer classification and diagnosis.

Author information

1
Adelaide Proteomics Centre, School of Molecular and Biomedical Science, The University of Adelaide, SA 5005, Adelaide, Australia; E-Mails: ove.gustafsson@adelaide.edu.au (J.O.R.G.); shaun.mccoll@adelaide.edu.au (S.R.M.).

Abstract

MALDI imaging mass spectrometry (MALDI-IMS) allows acquisition of mass data for metabolites, lipids, peptides and proteins directly from tissue sections. IMS is typically performed either as a multiple spot profiling experiment to generate tissue specific mass profiles, or a high resolution imaging experiment where relative spatial abundance for potentially hundreds of analytes across virtually any tissue section can be measured. Crucially, imaging can be achieved without prior knowledge of tissue composition and without the use of antibodies. In effect MALDI-IMS allows generation of molecular data which complement and expand upon the information provided by histology including immuno-histochemistry, making its application valuable to both cancer biomarker research and diagnostics. The current state of MALDI-IMS, key biological applications to ovarian cancer research and practical considerations for analysis of peptides and proteins on ovarian tissue are presented in this review.

KEYWORDS:

MALDI; biomarker; grading; imaging; mass spectrometry; ovarian cancer

PMID:
21340013
PMCID:
PMC3039979
DOI:
10.3390/ijms12010773
[Indexed for MEDLINE]
Free PMC Article

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