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J Surg Oncol. 2011 Mar 1;103(3):283-9. doi: 10.1002/jso.21802. Epub 2010 Dec 8.

Aberrant expression of USP22 is associated with liver metastasis and poor prognosis of colorectal cancer.

Author information

1
Department of Colorectal Surgery, The Affiliated Tumor Hospital of Harbin Medical University, Harbin, PR China.

Abstract

BACKGROUND:

The present study was aimed at clarifying the expression of ubiquitin carboxyl-terminal hydrolase 22 (USP22), a novel deubiquitinating enzyme gene, in colorectal cancer (CRC) and its clinical significance.

METHODS:

USP22 expression was detected with quantitative RT-PCR, Western blot, and immunohistochemistry (IHC) in 43 CRCs and non-cancerous matched tissues. Furthermore, USP22 protein expression was analyzed in 192 CRC tumors by IHC to evaluate the association with survival.

RESULTS:

In 43 paired fresh tissues, the expression level of USP22 was significantly higher in primary CRCs than that in the paired non-cancerous tissues at both mRNA and protein levels (P < 0.0001). Nuclear USP22 expression significantly increased from normal mucosa through adenoma to primary carcinoma (P < 0.0001) and from primary carcinoma to liver metastasis (P = 0.021). The incidence of positive USP22 expression was 54.16% in 192 conventional CRC tissues. Notably, high USP22 expression was significantly associated with shorter disease-specific survival (P < 0.0001) and shorter disease-free survival (P < 0.0001). Cox regression analysis showed USP22 was an independent prognostic parameter for CRC patients.

CONCLUSION:

USP22 might be an independent predictive factor for CRC prognosis and aberrant expression of USP22 may play an essential role in colorectal carcinogenesis and liver metastasis.

PMID:
21337558
DOI:
10.1002/jso.21802
[Indexed for MEDLINE]

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