Format

Send to

Choose Destination
EMBO Rep. 2011 Mar;12(3):231-7. doi: 10.1038/embor.2011.8. Epub 2011 Feb 18.

A model for the topology of active ribosomal RNA genes.

Author information

1
Science Faculty, Department of Molecular Biology, Nijmegen Centre for Molecular Life Sciences, Radboud University (274), PO Box 9101, Nijmegen 6500 HB, The Netherlands.

Abstract

The Christmas tree view of active ribosomal RNA (rRNA) genes suggests a gene topology in which a large number of nascent rRNA transcripts are prevented from intertwining. The way in which this is achieved has remained unclear. By using a combination of chromatin immunoprecipitation and chromosome conformation capture techniques, we show that the promoter, upstream region and terminator R3 of active rRNA genes are held together spatially throughout the cell cycle, forming a stable core around which the transcribed region is organized. We suggest a new core-helix model for the topology of rRNA genes, that provides a structural basis for the productive synthesis or rRNA.

PMID:
21331097
PMCID:
PMC3059908
DOI:
10.1038/embor.2011.8
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Wiley Icon for PubMed Central
Loading ...
Support Center