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Gut Microbes. 2010 Jul;1(4):209-212. Epub 2010 Jul 13.

Proinflammatory fecal mRNA and childhood bacterial enteric infections.

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1
Washington University School of Medicine; Department of Pediatrics; Division of Pediatric Gastroenterology and Nutrition; St. Louis, MO USA.

Abstract

INTRODUCTION:

Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied.

RESULTS:

Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin.

DISCUSSION:

Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA.

METHODS:

We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

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