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Gut Microbes. 2010 Jul;1(4):209-212. Epub 2010 Jul 13.

Proinflammatory fecal mRNA and childhood bacterial enteric infections.

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Washington University School of Medicine; Department of Pediatrics; Division of Pediatric Gastroenterology and Nutrition; St. Louis, MO USA.



Assessment of specific mRNAs in human samples is useful in characterizing disease. However, mRNA in human stool has been understudied.


Compared to controls, infected stools showed increased transcripts of IL-1β, IL-8 and calprotectin. mRNA and protein concentrations correlated for IL-8, but not for calprotectin.


Stool mRNA quantification offers a potentially useful, noninvasive way to assess inflammation in the gastrointestinal tract, and may be more sensitive than EIA.


We purified fecal RNA from 46 children infected with Campylobacter jejuni, Escherichia coli O157:H7, Salmonella spp. or Shigella sonnei and 26 controls and compared the proportions of IL-1β, IL-8, osteoprotegerin and calprotectin mRNA between groups using qRT-PCR. We determined the concentrations of calprotectin, IL-8 and osteoprotegerin by enzyme immunoassays in cognate specimens.

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