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J Clin Microbiol. 2011 Apr;49(4):1475-82. doi: 10.1128/JCM.02365-10. Epub 2011 Feb 16.

International external quality assurance for laboratory identification and typing of Streptococcus agalactiae (Group B streptococci).

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1
Streptococcus and Diphtheria Reference Unit, Respiratory and Systemic Infection Laboratory, Health Protection Agency, Microbiology Services Division, 61 Colindale Avenue, London NW9 5EQ, United Kingdom. Baharak.Afshar@hpa.org.uk

Abstract

We report the results from the first international multicenter external quality assessment (EQA) studies for molecular and serological typing of group B streptococcus (GBS) strains as part of DEVANI (Design of a Vaccine against Neonatal Infections), a pan-European program. A questionnaire-based surveillance was undertaken among eight laboratories participating in DEVANI and six laboratories not participating in DEVANI from 13 countries in order to assess their current microbiological procedures for GBS screening, diagnosis, and typing. GBS strains from three EQA distributions were characterized using molecular and serological methods based on GBS capsular polysaccharide typing. Participants were asked to test the first distribution using their current serotyping and genotyping methods. The Strep-B-Latex agglutination method was the most widely used method, with a typeability value of >90%. A multiplex PCR assay for GBS capsular gene typing was also used by 2 of 14 centers, which achieved a typeability value of 93%; this assay detected only 9 of 10 GBS capsular polysaccharide genes. From the second and third EQA studies, standardized protocols were prepared for serological and molecular typing of GBS strains based on the Strep-B-Latex agglutination method and a novel multiplex PCR assay that detected all 10 GBS capsular types (Ia to IX). These standardized protocols are being used by many European laboratories, and as the use of these methods increases, it is imperative to continuously improve and assess laboratory performance and offer training to any laboratories that have technical difficulties.

PMID:
21325542
PMCID:
PMC3122801
DOI:
10.1128/JCM.02365-10
[Indexed for MEDLINE]
Free PMC Article
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