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Glycobiology. 2011 Jul;21(7):895-902. doi: 10.1093/glycob/cwr021. Epub 2011 Feb 16.

Rapid characterization of sugar-binding specificity by in-solution proximity binding with photosensitizers.

Author information

1
Department of Medical Laboratory Science and Biotechnology, National Taiwan University, Taipei.

Abstract

Cell-surface carbohydrates are known to participate in many important physiological and pathological activities by interacting with their corresponding proteins or receptors. Although several methods have been developed for studying carbohydrate-protein interactions, one major problem originates from the weak bindings of carbohydrates/proteins that are often lost during repeating wash steps. Herein, we established a homogeneous solution carbohydrate array in which polyacrylamide-based glycans are used for offering a multivalent environment. The method requires no wash step and can be carried out in a high-throughput manner. We characterized the carbohydrate-binding specificities of 11 lectins and 7 antibodies, the majority of which displayed the binding patterns in consistence with previous reports. These results demonstrate that our developed solution carbohydrate array provides a useful alternative that is better than or comparable with the current available methods.

PMID:
21325337
DOI:
10.1093/glycob/cwr021
[Indexed for MEDLINE]

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