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J Microbiol Methods. 2011 May;85(2):164-9. doi: 10.1016/j.mimet.2011.01.027. Epub 2011 Feb 12.

Propidium monoazide combined with real-time quantitative PCR underestimates heat-killed Listeria innocua.

Author information

1
Nofima Norconserv AS, Stavanger, Norway. trond.lovdal@nofima.no

Abstract

The combination of propidium monoazide (PMA) and quantitative real-time PCR (qPCR) significantly overestimated the fraction of viable Listeria innocua as compared to plate counts and confocal fluorescence microscopy. Our data imply that PMA-qPCR must be used with caution as an analytical tool for the differentiation between viable and dead bacteria.

PMID:
21324348
DOI:
10.1016/j.mimet.2011.01.027
[Indexed for MEDLINE]

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