Format

Send to

Choose Destination
J Biotechnol. 2011 Mar 20;152(3):77-86. doi: 10.1016/j.jbiotec.2011.02.003. Epub 2011 Feb 12.

Control of adhA and sucR expression by the SucR regulator in Corynebacterium glutamicum.

Author information

1
Institute of Microbiology and Biotechnology, University of Ulm, Albert-Einstein-Allee 11, 89069 Ulm, Germany.

Abstract

The alcohol dehydrogenase gene adhA in Corynebacterium glutamicum is subject to a complex carbon source-dependent regulation mediated by RamA, RamB and GlxR. In this study we identified SucR as the fourth transcriptional regulator involved in expression control of the adhA gene. SucR specifically binds to the adhA promoter and acts as transcriptional repressor independent of the carbon source used. Furthermore, we found that SucR negatively controls the expression of its own gene. This negative autoregulation is mediated by binding of SucR to at least one of four identified binding sites located in the promoter region of sucR. EMSA experiments and subsequent sequence analysis led to the identification of the SucR consensus binding sequence YYAACAWMAW. This binding motif is different from the binding site (ACTCTAGGGG) recently described for SucR in the promoter region of the sucCD operon. However, we were not able to detect a specific interaction of purified SucR protein with this motif present in the sucCD promoter region.

PMID:
21320555
DOI:
10.1016/j.jbiotec.2011.02.003
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center