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Epidemiol Infect. 2012 Jan;140(1):36-41. doi: 10.1017/S0950268811000021. Epub 2011 Feb 15.

The application of an enzyme-linked immunosorbent assay or an immunofluorescent assay test leads to different estimates of seroprevalence of Coxiella burnetii in the population.

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Jeroen Bosch Ziekenhuis, 's-Hertogenbosch, The Netherlands.


The diagnosis and epidemiological studies of Q fever depend on serology. Among the main methods employed are the enzyme-linked immunosorbent assay (ELISA) and the immunofluorescent assay test (IFAT). We show that two commercial assays representing the two methods with two different cut-off titres can lead to significant differences in diagnostic and seroprevalence estimates. This in turn emphasizes the need for a standardized gold method to compare the various assays; whether this standard is 'in-house' or commercially obtained.

[Indexed for MEDLINE]

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