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J Biophotonics. 2011 Jun;4(6):377-90. doi: 10.1002/jbio.201000122. Epub 2011 Feb 14.

From EosFP to mIrisFP: structure-based development of advanced photoactivatable marker proteins of the GFP-family.

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1
National Oceanography Centre, University of Southampton, Southampton SO143ZH, UK.

Abstract

Fluorescent proteins from the GFP family have become indispensable imaging tools in life sciences research. In recent years, a wide variety of these proteins were discovered in non-bioluminescent anthozoa. Some of them feature exciting new properties, including the possibility to change their fluorescence quantum yield and/or color by irradiating with light of specific wavelengths. These photoactivatable fluorescent proteins enable many interesting applications including pulse-chase experiments and super-resolution imaging. In this review, we discuss the development of advanced variants, using a structure-function based, molecular biophysics approach, of the photoactivatable fluorescent protein EosFP, which can be photoconverted from green to red fluorescence by ~400 nm light. A variety of applications are presented that demonstrate the versatility of these marker proteins in live-cell imaging.

PMID:
21319305
DOI:
10.1002/jbio.201000122
[Indexed for MEDLINE]

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