Format

Send to

Choose Destination
Mar Biotechnol (NY). 2011 Oct;13(5):992-1002. doi: 10.1007/s10126-011-9363-x. Epub 2011 Feb 12.

A new coral carbonic anhydrase in Stylophora pistillata.

Author information

1
Centre Scientifique de Monaco, Avenue Saint Martin, 98000, Monaco, Monaco.

Abstract

Scleractinian corals are of particular interest due to their ability to establish an intracellular mutualistic symbiosis with phototrophic dinoflagellates and to deposit high rates of calcium carbonate in their skeleton. Carbonic anhydrases have been shown to play a crucial role in both processes. In this study, we report the molecular cloning and characterization of a novel α-CA in the coral Stylophora pistillata. This enzyme shares homologies with the human isoform CA II and is referred to as STPCA-2. STPCA-2 is 35.2 kDa and possesses all key amino acids for catalytic activity. With a ratio between catalytic and Michaelis constants (k(cat)/K(m)) of 8.3.10(7) M(-1)  s(-1) is considered as highly active. Owing to its intracellular localisation in the oral endoderm and in the aboral tissue, we propose that STPCA-2 is involved in pH regulation and/or inorganic carbon delivery to symbiont and calcification.

PMID:
21318259
DOI:
10.1007/s10126-011-9363-x
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Springer
Loading ...
Support Center