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J Biol Chem. 2011 Apr 15;286(15):13235-43. doi: 10.1074/jbc.M110.206433. Epub 2011 Feb 11.

Expanding the nucleotide and sugar 1-phosphate promiscuity of nucleotidyltransferase RmlA via directed evolution.

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1
Department of Biochemistry, University of Wisconsin, Madison, Wisconsin 53706, USA.

Abstract

Directed evolution is a valuable technique to improve enzyme activity in the absence of a priori structural knowledge, which can be typically enhanced via structure-guided strategies. In this study, a combination of both whole-gene error-prone polymerase chain reaction and site-saturation mutagenesis enabled the rapid identification of mutations that improved RmlA activity toward non-native substrates. These mutations have been shown to improve activities over 10-fold for several targeted substrates, including non-native pyrimidine- and purine-based NTPs as well as non-native D- and L-sugars (both α- and β-isomers). This study highlights the first broadly applicable high throughput sugar-1-phosphate nucleotidyltransferase screen and the first proof of concept for the directed evolution of this enzyme class toward the identification of uniquely permissive RmlA variants.

PMID:
21317292
PMCID:
PMC3075670
DOI:
10.1074/jbc.M110.206433
[Indexed for MEDLINE]
Free PMC Article
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