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Microb Cell Fact. 2011 Feb 11;10:8. doi: 10.1186/1475-2859-10-8.

Loosenin, a novel protein with cellulose-disrupting activity from Bjerkandera adusta.

Author information

1
Laboratorio de Biología Molecular de Hongos, Centro de Investigación en Biotecnología, Universidad Autónoma del Estado de Morelos, Avenida Universidad 1001 Col, Chamilpa, Cuernavaca 62209, Morelos, Mexico.

Abstract

BACKGROUND:

Expansins and expansin-like proteins loosen cellulose microfibrils, possibly through the rupture of intramolecular hydrogen bonds. Together with the use of lignocellulolytic enzymes, these proteins are potential molecular tools to treat plant biomass to improve saccharification yields.

RESULTS:

Here we describe a new type of expansin-related fungal protein that we have called loosenin. Its corresponding gene, loos1, from the basidiomycete Bjerkandera adusta, was cloned and heterologously expressed in Saccharomyces cerevisiae. LOOS1 is distantly related to plant expansins through the shared presence of a DPBB domain, however domain II found in plant expansins is absent. LOOS1 binds tightly to cellulose and chitin, and we demonstrate that cotton fibers become susceptible to the action of a commercial cellulase following treatment with LOOS1. Natural fibers of Agave tequilana also become susceptible to hydrolysis by cellulases after loosenin treatment.

CONCLUSIONS:

LOOS1 is a new type of protein with disrupting activity on cellulose. LOOS1 binds polysaccharides, and given its enhancing properties on the action of hydrolytic enzymes, LOOS1 represents a potential additive in the production of fermentable sugars from lignocellulose.

PMID:
21314954
PMCID:
PMC3050684
DOI:
10.1186/1475-2859-10-8
[Indexed for MEDLINE]
Free PMC Article

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