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PLoS One. 2011 Feb 2;6(2):e14648. doi: 10.1371/journal.pone.0014648.

Overexpression of BMI-1 promotes cell growth and resistance to cisplatin treatment in osteosarcoma.

Author information

1
Department of Orthopaedics, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.

Abstract

BACKGROUND:

BMI-1 is a member of the polycomb group of genes (PcGs), and it has been implicated in the development and progression of several malignancies, but its role in osteosarcoma remains to be elucidated.

METHODOLOGY/PRINCIPAL FINDINGS:

In the present study, we found that BMI-1 was overexpressed in different types of osteosarcomas. Downregulation of BMI-1 by lentivirus mediated RNA interference (RNAi) significantly impaired cell viability and colony formation in vitro and tumorigenesis in vivo of osteosarcoma cells. BMI-1 knockdown sensitized cells to cisplatin-induced apoptosis through inhibition of PI3K/AKT pathway. Moreover, BMI-1-depletion-induced phenotype could be rescued by forced expression of BMI-1 wobble mutant which is resistant to inhibition by the small interfering RNA (siRNA).

CONCLUSIONS/SIGNIFICANCE:

These findings suggest a crucial role for BMI-1 in osteosarcoma pathogenesis.

PMID:
21311599
PMCID:
PMC3032734
DOI:
10.1371/journal.pone.0014648
[Indexed for MEDLINE]
Free PMC Article

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