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Colloids Surf B Biointerfaces. 2011 Jun 1;84(2):301-9. doi: 10.1016/j.colsurfb.2011.01.012. Epub 2011 Jan 20.

Optimized grafting of antimicrobial peptides on stainless steel surface and biofilm resistance tests.

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Laboratoire de Réactivité de Surface, UMR CNRS 7197, Université Pierre et Marie Curie, UPMC Paris 6, 4 place Jussieu, 75252 Paris Cedex 05, France.


Antibacterial peptides, magainin I and nisin were covalently bound to stainless steel surfaces. Several procedures of surface functionalisation processes have been investigated and optimized, each step being characterized by polarization modulation reflection absorption infrared spectroscopy (PM-RAIRS) and X-ray photoemission spectroscopy (XPS). Grafting of antibacterial peptides was successfully achieved by a 3 steps functionalisation process on a chitosan polymeric layer. The antibacterial activity of the anchored magainin and nisin was tested against a gram-positive bacteria, Listeria ivanovii, i.e., the possible survival and attachment of this bacteria, was characterized on modified stainless steel surfaces. The results revealed that the adsorbed peptides reduced the adhesion of bacteria on the functionalised stainless steel surface.

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