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Development. 2011 Mar;138(5):831-7. doi: 10.1242/dev.057901.

Asymmetric division of cyst stem cells in Drosophila testis is ensured by anaphase spindle repositioning.

Author information

1
Department of Biomedical Engineering, Center for Ultrafast Optical Science, 1101 Beal Ave., Ann Arbor, MI 48109, USA.

Abstract

Many stem cells divide asymmetrically to balance self-renewal and differentiation. In Drosophila testes, two stem cell populations, germline stem cells (GSCs) and somatic cyst stem cells (CySCs), cohere and regulate one another. Here, we report that CySCs divide asymmetrically through repositioning the mitotic spindle around anaphase. CySC spindle repositioning requires functional centrosomes, Dynein and the actin-membrane linker Moesin. Anaphase spindle repositioning is required to achieve high-fidelity asymmetric divisions in CySCs, thus maintaining both GSC and CySC numbers. We propose that dynamic spindle repositioning allows CySCs to divide asymmetrically while accommodating the structure of the GSCs they encapsulate.

PMID:
21303845
PMCID:
PMC3035088
DOI:
10.1242/dev.057901
[Indexed for MEDLINE]
Free PMC Article

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