Format

Send to

Choose Destination
Angle Orthod. 2011 Jul;81(4):592-9. doi: 10.2319/090310-519.1. Epub 2011 Feb 7.

Mechanical loading activates β-catenin signaling in periodontal ligament cells.

Author information

1
Orthodontic Section, Department of Growth and Development, University of Nebraska Medical Center College of Dentistry, Lincoln, NE 68583, USA. spremaraj@unmc.edu

Abstract

OBJECTIVE:

To determine whether β-catenin signaling is responsive to mechanical loading in periodontal ligament (PDL) cells.

MATERIALS AND METHODS:

To determine whether Wnt/β-catenin signaling pathway components are present and functional, PDL cells were treated with lithium chloride or Wnt3a-conditioned media. To determine whether mechanical strain activates β-catenin signaling, PDL cells were subjected to compressive loading. Activation of the β-catenin signaling pathway was determined by immunofluorescence, Western immunoblotting, and TOPflash assay.

RESULTS:

Mimicking Wnt signaling stimulates β-catenin nuclear translocation and T-cell factor/lymphoid enhancer binding factor-dependent transcriptional activation in PDL cells. Mechanical loading stimulates a transient accumulation of dephosphorylated β-catenin in the cytoplasm and its translocation to the nucleus. This effect of strain acts through activation of protein kinase B and phosphorylation of glycogen synthase kinase-3 beta. These strain-related changes do not involve the low-density lipoprotein receptor-related protein 5/Wnt receptor.

CONCLUSIONS:

The Wnt/β-catenin signaling pathway components are functional and activated by mechanical loading in PDL cells. β-catenin serves as an effector of mechanical signals in PDL cells.

PMID:
21299429
DOI:
10.2319/090310-519.1
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Allen Press, Inc.
Loading ...
Support Center