Format

Send to

Choose Destination
Nat Methods. 2011 Mar;8(3):242-5. doi: 10.1038/nmeth.1569. Epub 2011 Feb 6.

High-throughput single-molecule optofluidic analysis.

Author information

1
Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles, California, USA.

Abstract

We describe a high-throughput, automated single-molecule measurement system, equipped with microfluidics. The microfluidic mixing device has integrated valves and pumps to accurately accomplish titration of biomolecules with picoliter resolution. We demonstrate that the approach enabled rapid sampling of biomolecule conformational landscape and of enzymatic activity, in the form of transcription by Escherichia coli RNA polymerase, as a function of the chemical environment.

Comment in

PMID:
21297618
PMCID:
PMC3075913
DOI:
10.1038/nmeth.1569
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for Nature Publishing Group Icon for PubMed Central
Loading ...
Support Center