Format

Send to

Choose Destination
See comment in PubMed Commons below
Vet Microbiol. 2011 May 12;150(1-2):146-51. doi: 10.1016/j.vetmic.2010.12.025. Epub 2011 Jan 11.

Intracellular Yersinia pestis expresses general stress response and tellurite resistance proteins in mouse macrophages.

Author information

1
Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078, USA.

Abstract

Yersinia pestis inoculated subcutaneously via fleabite or experimental injection in natural rodent hosts multiply initially in macrophage phagolysosomes. Survival and multiplication of Y. pestis in this acidic low [Ca(2+)] and [Mg(2+)] environment likely necessitates compensatory mechanisms involving expression of specific proteins compared to those expressed during extracellular growth. A proteomics approach was used to identify these proteins using mouse macrophage RAW264.7 cells infected with Y. pestis strain KIM6-2053.1+ for 8h. Intracellular Y. pestis protein samples were prepared by detergent lysis of infected RAW264.7 cells, isolation of intracellular Y. pestis by differential centrifugation, and sonication of isolated Y. pestis. Protein samples were similarly prepared from Y. pestis grown extracellularly in tissue culture media. Two intracellular and extracellular Y. pestis protein samples were analyzed by two-dimensional polyacrylamide gel electrophoresis and compared in silico identifying 12 protein spots present in both intracellular samples but absent in extracellularly grown Y. pestis. Mass spectrometry analysis of these identified nine proteins at a high level of confidence in the Y. pestis genome: superoxide dismutase-A (sodA), inorganic pyrophosphatase, autonomous glycyl radical cofactor GrcA, molecular chaperone DnaK, serine endoprotease GsrA, global DNA-binding transcriptional dual regulator H-NS, urease subunit gamma UreA, and tellurite resistance proteins TerD and TerE. These results support the involvement of various general stress response regulators of Y. pestis during the intracellular parasitism of host macrophages as well as identification of UreA, TerD and TerE with as yet unknown roles in the process of intracellular survival of Y. pestis.

PMID:
21295415
DOI:
10.1016/j.vetmic.2010.12.025
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center