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Small. 2011 Feb 7;7(3):364-70. doi: 10.1002/smll.201001314. Epub 2010 Dec 30.

Effective gene silencing by multilayered siRNA-coated gold nanoparticles.

Author information

1
Department of Radiology, The Methodist Hospital Research Institute, Weill Cornell Medical College, 6565 Fannin St. B5-009, Houston, TX 77030, USA.

Abstract

Small interfering RNA (siRNA) has been widely proposed to treat various diseases by silencing genes, but its delivery remains a challenge. A well controlled assembly approach is applied to prepare a protease-assisted nanodelivery system. Protease-degradable poly-L-lysine (PLL) and siRNA are fabricated onto gold nanoparticles (AuNPs), by alternating the charged polyelectrolytes. In this study, up to 4 layers of PLL and 3 layers of siRNA (sR3P) are coated. Due to the slow degradation of PLL, the incorporated siRNA is released gradually and shows extended gene-silencing effects. Importantly, the inhibition effect in cells is found to correlate with the number of siRNA layers.

KEYWORDS:

delivery; gold nanoparticles; multilayer siRNA; poly-L-lysine; siRNA

PMID:
21294265
PMCID:
PMC3099143
DOI:
10.1002/smll.201001314
[Indexed for MEDLINE]
Free PMC Article
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