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Emerg Infect Dis. 2011 Feb;17(2):296-8. doi: 10.3201/eid1702.101123.

Usefulness of published PCR primers in detecting human rhinovirus infection.

Author information

1
The University of Queensland, Brisbane, Queensland, Australia.

Abstract

We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.

PMID:
21291610
PMCID:
PMC3204776
DOI:
10.3201/eid1702.101123
[Indexed for MEDLINE]
Free PMC Article

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