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J Clin Microbiol. 2011 Apr;49(4):1363-8. doi: 10.1128/JCM.02403-10. Epub 2011 Feb 2.

Combining culture techniques for Bartonella: the best of both worlds.

Author information

1
Centers for Disease Control and Prevention, Division of Vector Borne Diseases, 3150 Rampart Road, Fort Collins, CO 80521, USA.

Abstract

In this study we compared some common Bartonella culturing methodologies using four diverse species causing human illnesses. Based on a review of the literature, we focused on three major inconsistencies between protocols: base medium, cell coculture, and temperature. Our data showed that Bartonella tamiae demonstrated temperature-dependent growth limitations between common culturing conditions only 2°C apart. Additionally, growth of B. quintana was significantly enhanced by the presence of mammalian cell coculture under mammalian cell culture conditions; however, when the medium was modified to incorporate insect cell culture-based medium, coculturing with mammalian cells was no longer needed. In this study, we were able to overcome these temperature- and cell-dependent limitations and accommodate all of the strains tested by combining mammalian cell culture-based medium with insect cell culture-based medium.

PMID:
21289156
PMCID:
PMC3122786
DOI:
10.1128/JCM.02403-10
[Indexed for MEDLINE]
Free PMC Article

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