Send to

Choose Destination
Eur J Obstet Gynecol Reprod Biol. 2011 May;156(1):96-100. doi: 10.1016/j.ejogrb.2010.12.023. Epub 2011 Feb 1.

Type-specific human papillomavirus DNA testing with the genotyping array: a comparison of cervical and vaginal sampling.

Author information

Department of Obstetrics and Gynecology, Taipei Veterans General Hospital, Taiwan.



To compare the detection and typing of human papillomavirus (HPV) between vaginal and cervical specimens by using polymerase chain reaction (PCR)-based reverse-blot genotyping arrays.


Two hundred and fifty-two women were referred to colposcopy clinics because of suspicious or positive results in a community-based cervical cancer-screening program. Genital tract cells were sampled from the cervix and self-collected from the vagina and tested with the HPV Blot kit.


The HPV Blot kit identified HPV infection in 24.7% of vaginal specimens and in 30.2% of cervical collections. Cervical sampling detected significantly more infections compared to vaginal sampling only for HPV type 52; cervical sampling also detected significantly more high-risk HPV infection overall. The sensitivities of detecting histology ≥ cervical intraepithelial neoplasia (CIN) grade 3 using the HPV Blot in vaginal and cervical specimens were 75.0% (95% CI, 47.6-92.7%) and 87.5% (95% CI, 61.6-98.4%), respectively (P=0.48). Both sampling methods were thus statistically effective at detecting high-grade lesions and cervical cancer (P<0.0001).


The HPV Blot yielded similar results for both vaginal sampling and cervical sampling in the detection of CIN grade 3 or worse. These findings indicate that self-sampling for HPV testing is a viable cervical cancer screening option.

[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center