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Proc Natl Acad Sci U S A. 2011 Feb 15;108(7):2729-34. doi: 10.1073/pnas.1010721108. Epub 2011 Jan 31.

Thiol peroxidases mediate specific genome-wide regulation of gene expression in response to hydrogen peroxide.

Author information

1
Department of Biochemistry, University of Nebraska, Lincoln, NE 68588-0664, USA.

Abstract

Hydrogen peroxide is thought to regulate cellular processes by direct oxidation of numerous cellular proteins, whereas antioxidants, most notably thiol peroxidases, are thought to reduce peroxides and inhibit H(2)O(2) response. However, thiol peroxidases have also been implicated in activation of transcription factors and signaling. It remains unclear if these enzymes stimulate or inhibit redox regulation and whether this regulation is widespread or limited to a few cellular components. Herein, we found that Saccharomyces cerevisiae cells lacking all eight thiol peroxidases were viable and withstood redox stresses. They transcriptionally responded to various redox treatments, but were unable to activate and repress gene expression in response to H(2)O(2). Further studies involving redox transcription factors suggested that thiol peroxidases are major regulators of global gene expression in response to H(2)O(2). The data suggest that thiol peroxidases sense and transfer oxidative signals to the signaling proteins and regulate transcription, whereas a direct interaction between H(2)O(2) and other cellular proteins plays a secondary role.

PMID:
21282621
PMCID:
PMC3041109
DOI:
10.1073/pnas.1010721108
[Indexed for MEDLINE]
Free PMC Article

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