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Nat Med. 2011 Feb;17(2):175-8. doi: 10.1038/nm.2294. Epub 2011 Jan 30.

Ultrasensitive human prion detection in cerebrospinal fluid by real-time quaking-induced conversion.

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  • 1Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences, Nagasaki, Japan. atarashi@nagasaki-u.ac.jp

Abstract

The development of technologies for the in vitro amplification of abnormal conformations of prion protein (PrP(Sc)) has generated the potential for sensitive detection of prions. Here we developed a new PrP(Sc) amplification assay, called real-time quaking-induced conversion (RT-QUIC), which allows the detection of ≥1 fg of PrP(Sc) in diluted Creutzfeldt-Jakob disease (CJD) brain homogenate. Moreover, we assessed the technique first in a series of Japanese subjects and then in a blind study of 30 cerebrospinal fluid specimens from Australia, which achieved greater than 80% sensitivity and 100% specificity. These findings indicate the promising enhanced diagnostic capacity of RT-QUIC in the antemortem evaluation of suspected CJD.

PMID:
21278748
DOI:
10.1038/nm.2294
[PubMed - indexed for MEDLINE]
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