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J Gen Microbiol. 1990 Dec;136(12):2521-6.

Determination of turgor pressure in Bacillus subtilis: a possible role for K+ in turgor regulation.

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Department of Biological Sciences, University of Dundee, UK.


The effects of hypersaline treatment (osmotic upshock) on cell water relations were examined in the Gram-positive bacterium Bacillus subtilis using particle size analysis. Application of the Boyle-van't Hoff relationship (cell volume versus reciprocal of external osmolality) permitted direct determination of turgor pressure, which was approximately 0.75 osmol kg-1 (1.9 MPa) in exponentially growing bacteria in a defined medium. The abolition of turgor pressure immediately after upshock and the subsequent recovery of turgor were investigated. Recovery of turgor was K+ dependent. Calculation of turgor by an alternative method involving spectrophotometric analysis of shrinkage gave somewhat lower estimates of turgor pressure.

[Indexed for MEDLINE]

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