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Cancer Sci. 2011 May;102(5):1059-67. doi: 10.1111/j.1349-7006.2011.01893.x. Epub 2011 Mar 7.

Resveratrol as an adjunct therapy in cyclophosphamide-treated MCF-7 cells and breast tumor explants.

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1
Tissue and Cell Culture Unit, Central Drug Research Institute, CSIR, Lucknow, India. neetuaashi@yahoo.com

Abstract

Cyclophosphamide (CPA) has efficacy as a breast cancer therapy. However, toxicity to CPA limits its clinical applications. Hence there is a need to develop compounds that may be combined with it to improve the efficacy and overcome toxicity. We showed previously that Resveratrol (RES), a chemopreventive agent, increased the growth inhibitory effect of CPA-treated MCF-7 cells. Here we have explored the molecular basis of 5 mM CPA and 50 μM RES as a combination on cell-cycle progression, apoptosis and oxidative stress in MCF-7 breast cancer cells. Efficacy of the combination was also evaluated in a serum-free tumor explant culture model. The combination elicited enhanced anti-proliferative action coupled with differential expression of cell-cycle, apoptosis and stress factors. Furthermore, co-treatment superiority in histologically validated ER positive breast cancer explants suggests that this combination may be a worthy future clinical anti-neoplastic regimen.

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