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J Am Chem Soc. 2011 Feb 23;133(7):2080-3. doi: 10.1021/ja110157u. Epub 2011 Jan 28.

Enzymatic deamination of the epigenetic base N-6-methyladenine.

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  • 1Department of Chemistry, Texas A&M University, P.O. Box 30012, College Station, Texas 77842-3012, United States.

Abstract

Two enzymes of unknown function from the amidohydrolase superfamily were discovered to catalyze the deamination of N-6-methyladenine to hypoxanthine and methyl amine. The methylation of adenine in bacterial DNA is a common modification for the protection of host DNA against restriction endonucleases. The enzyme from Bacillus halodurans, Bh0637, catalyzes the deamination of N-6-methyladenine with a k(cat) of 185 s(-1) and a k(cat)/K(m) of 2.5 × 10(6) M(-1) s(-1). Bh0637 catalyzes the deamination of N-6-methyladenine 2 orders of magnitude faster than adenine. A comparative model of Bh0637 was computed using the three-dimensional structure of Atu4426 (PDB code: 3NQB) as a structural template and computational docking was used to rationalize the preferential utilization of N-6-methyladenine over adenine. This is the first identification of an N-6-methyladenine deaminase (6-MAD).

PMID:
21275375
PMCID:
PMC3043370
DOI:
10.1021/ja110157u
[PubMed - indexed for MEDLINE]
Free PMC Article
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