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Anticancer Res. 2011 Jan;31(1):139-45.

Oncogramme, a new promising method for individualized breast tumour response testing for cancer treatment.

Author information

1
Oncomedics, ESTER Technopole, Limoges, France. s.giraud@oncomedics.com

Abstract

BACKGROUND:

Breast cancer is the most widely spread cancer in the world, attracting much research and individualized tumour response testing (ITRT) methods are now used to individualize patient chemotherapeutic administrations. A new ITRT method was developed with optimized processing.

MATERIALS AND METHODS:

Breast tumour fragments were separated and the cells seeded in a foetal calf serum-free defined medium. After various chemotherapeutic treatments, cytotoxicity was determined by cell death detection with calcein acetoxymethyl and ethidium homodimer labelling.

RESULTS:

The culture medium allowed breast tumour cell proliferation in culture, while preventing fibroblastic cell survival. Moreover, the cell death analysis gave rise to a chemoresistance profile called an Oncogramme, with statistically significant values.

CONCLUSION:

The Oncogramme is a new ITRT method which can predict patient cell sensitivities to chemotherapeutics and should be validated by a new phase I clinical trial.

PMID:
21273591
[Indexed for MEDLINE]

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